Development of plans family selection: genealogical traceability
Genetics provides the most powerful technology for the improvement of animal production. The family selection programs are based on the exploitation of the available genetic diversity within and between families to obtain the highest gain per generation. To do this, the crosses are designed between the breeders of each generation of selection according to their genetic value and always avoiding the harmful effects of the inbred crosses. For this, it is essential kinship knowledge among the individuals of the broodstock. Taking into account the usual mix of different families in the same tank before selection in fish farming, it is essential the development of molecular tools that make it possible to establish the genealogy for development of a selection program.
Monitoring and improving selection plans
Even in cases where families grow in individual, the use of molecular traceability tools are necessary for checking progenies of crosses designed into the plan. The large number of tasks and management involving selection programs at farm facilities makes necessary to check for handling errors for correction and improvement the performance of the selection program.
Development of breeding plans
The main target characters of genetic breeding programs in fish are related to growth, reproduction, diseases and morphological abnormalities. Understanding the genetic basis of these characters enables more efficient selection outcomes than those based only on kinship and phenotype of individuals. Genomics has been a breakthrough in this knowledge and provides genetic markers associated with growth, resistance to diseases, sex, etc. Marker-assisted selection is the selection of breeders that they have genetic variants of commercial interest and allows for greater efficiency in the selection process.
Service for the development of molecular markers for population-based studies or kinship
A) Development of microsatellite markers: from a run in the Illumina MiSeq sequencer around 30 million of 500bp sequences will be obtained. These will be used for tracking microsatellite motifs, designing of primers and validation of polymorphism in agarose gels and automatic sequencer (ABI 3730). This service may include all stages including the bioinformatic analysis with the hundreds of sequences containing microsatellite markers obtained. The user should provide only 10 ug of high quality DNA for sequencing.
B) Development of SNPs using RAD technology in an Illumina NextSeq 500 sequencer. The sequences obtained will be assembled through the use of bioinformatics programs to develop a database for location of SNPs. The service also includes the possibility of SNP validation and their combination in multiplexes.
Gene sequencing amplicons of interest and bacterial genomes
Gene sequencing amplicons of interest or gene clusters: From the amplicons sent by stakeholders, an Illumina MiSeq sequencing will be performed and the subsequent bioinformatic assembly will be performed using as reference the genes of the species of interest.
Sequencing of bacterial genomes: From genomic DNA of the species of interest an Illumina MiSeq sequencing will be run and subsequently assembled against the reference genome of the species of interest. Sequencing “de novo” can also be made. In this case the assembly will require more bioinformatic work and consequently a higher cost.
From the biological samples sent by stakeholders, a highly conserved fragment of the genome of the target species will be amplified and subsequently sequenced in the Illumina Miseq. Amplicon assembly using the genes of the species of interest as reference will allow their identification and quantification from the mixture of species in the sample.
- Provide on demand zebrafish embryos (different stages of development)
- Toxicity assays
- Xenotrasplantation assays to validate new drugs
- Genes Inactivation: morpholinos
- Creation of transgenic lines for the development of human models diseases
Customer services on request
The system allows services to be flexible and meet the needs of each client.